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The Space Bikers are a group of anthropomorphic fish alien bikers that at one point were staff members of a school on their home planet. They enjoy parties, and especially love to crash parties on Earth, wreaking mayhem and pranking and roughing-up humans in the process. They have trashed Mezmer's, Jenny's backyard crash pad, and Jenny's high school.

Jenny normally beats the Space Bikers with relative ease, but they have defeated her at least twice. One of these times, in Victim of Fashion, the Space Bikers wanted revenge against her. They captured her without much of a fight because Jenny had all her weapons removed so she could beat Brit and Tiff in a fashion war. Tuck rescued Jenny, Brad and Sheldon with a mech made from Jenny's removed and tossed aside weapons. The other time, in Voyage to the Planet of the Bikers, they beat Jenny and turn her into a motorcycle. Jenny wasn't able to transform back so, Jenny and Tuck went to the Space Bikers' planet to make them fix her. The Space Bikers lost their school staff jobs in the process.

The members of the Space Bikers include Letta, Olga, Sludge, Tammy and Lenny.