Vladimir (formerly known as Mr. Scruffles) was Nora Wakeman's former lab-rat and leader in a gang of rats who debuted in "Pest Control." Tired of being treated like a "pet", he exacted revenge on Nora by stealing Jenny's body. He was defeated when the body was knocked into a pool, to which his minions "abandoned ship" to avoid the water and electrocution. He was also the leader of The Legion of Evil. His voice was provided by Kevin Michael Richardson.
- Vladimir is the first antagonist of the entire series.
- Vladimir's design is reminiscent of the early incarnations of Mickey Mouse (Disney mascot), as well as Mickey's nemesis Mortimer Mouse.
- The name, personality, and plot of the story are a reference the the Russian Revolution in 1917, and Vladimir Lenin ( the exact name of Mr. Scruffles), the famous revolutionary leader - Vladimir says things like "Comrades" (A commonly known Russian term for "friends") and "we have suffered much under the cruelty of Dr. Wakeman" (the exact same scenario the Russians faced under the leadership of Nicholas II). Vladmir's personality is commanding, like the real Vladimir, and the plot (Spoiler in "Pest Control") of the mice (Russians) getting hold of XJ-9's body (weaponry), but were easily infuriated by Brad, Tuck, Jenny, and Mrs. Wakeman (Americans), and because of the mice's instincts (Cowardice in the face of fear) leading Vladmir to fail and the mice getting caught and taken away by the Pest Control service (Collapse of the U.S.S.R).